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Novel transcript discovery in yeast using sequencing reads from Oxford Nanopore Technologies in comparison to Next Generation Sequencing

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dc.contributor Universitat de Vic - Universitat Central de Catalunya. Facultat de Ciències i Tecnologia
dc.contributor Universitat de Vic - Universitat Central de Catalunya. Màster Universitari en Anàlisi de Dades Òmiques
dc.contributor.author Lloret-Villas, Audald
dc.date.accessioned 2019-03-14T18:05:27Z
dc.date.available 2019-03-14T18:05:27Z
dc.date.created 2018-09
dc.date.issued 2018-09
dc.identifier.uri http://hdl.handle.net/10854/5738
dc.description Curs 2017-2018 es
dc.description.abstract The advent of Oxford Nanopore Technologies is expected to play a pivotal role in near future sequencing studies. Omics data analysis and related processes, currently bound to Next Generation Sequencing, can be now complemented and improved by the rise of Nanopore devices, tools and pipelines. We have herein explored the advantages and limitations of the application of Nanopore technologies for the reconstruction of transcriptomes as well as the discovery of unannotated genes sequences in yeast. Taking Saccharomyces cerevisiae as our focal species, comprehensive pipelines have been developed, optimised and compared with other processing approaches, also generated in-house. The comparisons of newly suggested pipelines with existing Illumina-based strategies are of especial interest. As a result of this investigation project, we provide insights about the impact of different technologies and pipelines for transcriptomics data processing and analysis. At the same time, reproducible code and technical details are made available along with the report. es
dc.format application/pdf es
dc.format.extent 40 p. es
dc.language.iso eng es
dc.rights Tots els drets reservats es
dc.subject.other Bioinformàtica es
dc.subject.other Genòmica es
dc.subject.other Transcriptomes es
dc.subject.other Expressió gènica es
dc.title Novel transcript discovery in yeast using sequencing reads from Oxford Nanopore Technologies in comparison to Next Generation Sequencing es
dc.type info:eu-repo/semantics/masterThesis es
dc.rights.accesRights info:eu-repo/semantics/closedAccess es

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