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Integrating quantitative proteomics and metabolomics in a cellular model of diabetic retinopathy

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dc.contributor Universitat de Vic. Escola Politècnica Superior
dc.contributor Universitat de Vic. Màster Universitari en Anàlisi de Dades Òmiques
dc.contributor.author Capellades Tomàs, Jordi
dc.date.accessioned 2014-10-22T08:36:58Z
dc.date.available 2014-10-22T08:36:58Z
dc.date.created 2014-09
dc.date.issued 2014-09
dc.identifier.uri http://hdl.handle.net/10854/3456
dc.description Curs 2013-2014 ca_ES
dc.description.abstract Diabetic retinopathy is the leading cause of visual loss in individuals under the age of 55. Most investigations into the pathogenesis of diabetic retinopathy have been concentrated on the neural retina since this is where clinical lesions are manifested. Recently, however, various abnormalities in the structural and secretory functions of retinal pigment epithelium that are essential for neuroretina survival, have been found in diabetic retinopathy. In this context, here we study the effect of hyperglycemic and hypoxic conditions on the metabolism of a human retinal pigment epithelial cell line (ARPE-19) by integrating quantitative proteomics using tandem mass tagging (TMT), untargeted metabolomics using MS and NMR, and 13C-glucose isotopic labeling for metabolic tracking. We observed a remarkable metabolic diversification under our simulated in vitro hyperglycemic conditions of diabetes, characterized increased flux through polyol pathways and inhibition of the Krebs cycle and oxidative phosphorylation. Importantly, under low oxygen supply RPE cells seem to consume rapidly glycogen storages and stimulate anaerobic glycolysis. Our results therefore pave the way to future scenarios involving new therapeutic strategies addressed to modulating RPE metabolic impairment, with the aim of regulating structural and secretory alterations of RPE. Finally, this study shows the importance of tackling biomedical problems by integrating metabolomic and proteomics results. ca_ES
dc.format application/pdf
dc.format.extent 23 p. ca_ES
dc.language.iso cat ca_ES
dc.rights Aquest document està subjecte a aquesta llicència Creative Commons ca_ES
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/es/ ca_ES
dc.subject.other Retinopatia diabètica ca_ES
dc.title Integrating quantitative proteomics and metabolomics in a cellular model of diabetic retinopathy ca_ES
dc.type info:eu-repo/semantics/masterThesis ca_ES
dc.description.version Director/a: Oscar Yanes Torrado, Jordi Planas Cuchi
dc.rights.accesRights info:eu-repo/semantics/openAccess ca_ES

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