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Skewed expression and up-regulation of the IL-12 and IL-18 receptors in resting and activated CD4 T cells from HIV-1-infected patients

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dc.contributor Universitat de Vic. Escola Politècnica Superior
dc.contributor Universitat de Vic. Grup de Recerca en Bioinformàtica i Estadística Mèdica
dc.contributor.author De Arquer, Guillermo Robert
dc.contributor.author Peña, Ruth
dc.contributor.author Cabrera, Cecilia
dc.contributor.author Coma, Gemma
dc.contributor.author Ruiz Hernández, Raul
dc.contributor.author Guerola, Rosa
dc.contributor.author Clotet, Bonaventura
dc.contributor.author Ruiz, Lídia
dc.contributor.author Esté, José A.
dc.contributor.author Calle, M. Luz
dc.contributor.author Bofill, Margarita
dc.date.accessioned 2012-10-17T08:21:32Z
dc.date.available 2012-10-17T08:21:32Z
dc.date.created 2007
dc.date.issued 2007
dc.identifier.citation DE ARQUER, Guillermo Roberto i altres . "Skewed expression and up-regulation of the IL-12 and IL-18 receptors in resting and activated CD4 T cells from HIV-1-infected patients". A: Journal of leukocyte biology, june 29, 2007, vol. 82, núm. 1, pàg. 72-78. doi:10.1189/jlb.1106698 ca_ES
dc.identifier.issn 0741-5400
dc.identifier.uri http://hdl.handle.net/10854/1903
dc.description.abstract IL-12 and IL-18 synergistically induce the production of IFN- by resting and activated T cells. To evaluate whether this induction was affected in HIV-1-infected patients, PBMC or isolated CD4 T cells were cultured with IL-12 plus IL-18, anti-CD3 plus anti-CD28, or PHA for 72 h. Cell samples were labeled daily to assess the levels of IL-12 receptor 1 (IL-12R 1), IL-12R 2, and IL-18R . Culture supernatants were analyzed for the presence of Th1- and Th2-related cytokines by ELISA or cytometric bead array and analyzed by flow cytometry. A twofold increase in the percentage of CD4-resting T cells expressing IL-12R 1 and IL-18R from HIV-1-infected patients was observed when compared with cells from HIV-1-negative donors. Higher IL-12R 1 and IL-18R expression correlated (r 0.87; P<0.007) to increased production of IFN- by isolated CD4 T cells in the presence of IL-12 and IL-18. Moreover, exogenous IL-12 and IL-18 induced the upregulation of IL-12R 2 to twice higher in CD4 T cells from HIV-1-positive individuals compared with controls. Conversely, upon activation with anti-CD3 and anti-CD28 antibodies, only 25% of the CD4 T cells from HIV-1 patients showed an increase in the IL-12 2 when compared with 50% in healthy controls. Furthermore, the percentage of IL-12R 1-positive cells correlated inversely with the CD4 nadir of patients, suggesting that deregulation of the IL-12 and IL-18 pathways may play a role in the immunopathogenesis of HIV-1 infection. J. Leukoc. Biol. 82: 72–78; 2007. ca_ES
dc.format application/pdf
dc.format.extent 7 p. ca_ES
dc.language.iso eng ca_ES
dc.publisher Society for Leukocyte Biology ca_ES
dc.rights (c) Society for Leukocyte Biology
dc.rights Tots els drets reservats ca_ES
dc.subject.other Bioinformàtica ca_ES
dc.title Skewed expression and up-regulation of the IL-12 and IL-18 receptors in resting and activated CD4 T cells from HIV-1-infected patients ca_ES
dc.type info:eu-repo/semantics/article ca_ES
dc.identifier.doi https://doi.org/doi:10.1189/jlb.1106698
dc.relation.publisherversion http://dx.doi.org/doi:10.1189/jlb.1106698
dc.rights.accesRights info:eu-repo/semantics/closedAccess
dc.type.version info:eu-repo/semantics/publishedVersion
dc.indexacio Indexat a SCOPUS
dc.indexacio Indexat a WOS/JCR ca_ES

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