Patient-derived induced pluripotent stem cells (iPSC) are a valuable approach to model cardiovascular diseases. We nucleofected non-integrating episomal vectors in
skin fibroblasts of three family members carrying a single nucleotide variant (SNV) in SCN5A, which encodes the cardiac-type sodium channel, and of a related
healthy ...»»»»
Patient-derived induced pluripotent stem cells (iPSC) are a valuable approach to model cardiovascular diseases. We nucleofected non-integrating episomal vectors in
skin fibroblasts of three family members carrying a single nucleotide variant (SNV) in SCN5A, which encodes the cardiac-type sodium channel, and of a related
healthy control. The SNV SCN5A_c.4573G > A had been previously identified in a Brugada Syndrome patient. The resulting iPS cell lines differentiate into cells of the
3 germ layers, display normal karyotypes and express pluripotency surface markers and genes. Thus, they are a reliable source to study the effect of the identified
mutation in a physiologically relevant environment.^^^^
Tipo de documento:
Artículo
Indexación:
Indexat a WOS/JCR
Indexat a SCOPUS
Citación Bibliográfica:
Martínez-Moreno, R., Pérez-Serra, A., Carreras, D., Aran, B., Kuebler, B., Brugada, R., Selga, E. (2022). Generation of an induced pluripotent stem cell line from a healthy caucasian male. Stem Cell Research, 60(102717). https://doi.org/10.1016/j.scr.2022.102717