The advent of Oxford Nanopore Technologies is expected to play a pivotal role in near future
sequencing studies. Omics data analysis and related processes, currently bound to Next
Generation Sequencing, can be now complemented and improved by the rise of Nanopore
devices, tools and pipelines.
We have herein explored the ...»»»»
The advent of Oxford Nanopore Technologies is expected to play a pivotal role in near future
sequencing studies. Omics data analysis and related processes, currently bound to Next
Generation Sequencing, can be now complemented and improved by the rise of Nanopore
devices, tools and pipelines.
We have herein explored the advantages and limitations of the application of Nanopore
technologies for the reconstruction of transcriptomes as well as the discovery of unannotated
genes sequences in yeast. Taking Saccharomyces cerevisiae as our focal species, comprehensive
pipelines have been developed, optimised and compared with other processing approaches, also
generated in-house. The comparisons of newly suggested pipelines with existing Illumina-based
strategies are of especial interest.
As a result of this investigation project, we provide insights about the impact of different
technologies and pipelines for transcriptomics data processing and analysis. At the same time,
reproducible code and technical details are made available along with the report.^^^^