Research Techniques Made Simple: Analysis of Collective Cell Migration Using the Wound Healing Assay

Grada, Ayman; Otero Viñas, Marta; Prieto-Castrillo, Francisco; Obagi, Zaidal; Falanga, V.

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dc.contributor	Universitat de Vic - Universitat Central de Catalunya. Facultat de Ciències i Tecnologia
dc.contributor.author	Grada, Ayman
dc.contributor.author	Otero Viñas, Marta
dc.contributor.author	Prieto-Castrillo, Francisco
dc.contributor.author	Obagi, Zaidal
dc.contributor.author	Falanga, V.
dc.date.accessioned	2017-02-21T08:38:20Z
dc.date.available	2017-02-21T08:38:20Z
dc.date.created	2017
dc.date.issued	2017
dc.identifier.citation	Grada, A., Otero-Vinas, M., Prieto-Castrillo, F., Obagi, Z., Falanga V. (2017). Research Techniques Made Simple: Analysis of Collective Cell Migration Using the Wound Healing Assay. Journal of Investigative Dermatology, 137(2), e11-e16.	es
dc.identifier.issn	1523-1747
dc.identifier.uri	http://hdl.handle.net/10854/4920
dc.description.abstract	Collective cell migration is a hallmark of wound repair, cancer invasion and metastasis, immune responses, angiogenesis, and embryonic morphogenesis. Wound healing is a complex cellular and biochemical process necessary to restore structurally damaged tissue. It involves dynamic interactions and crosstalk between various cell types, interaction with extracellular matrix molecules, and regulated production of soluble mediators and cytokines. In cutaneous wound healing, skin cells migrate from the wound edges into the wound to restore skin integrity. Analysis of cell migration in vitro is a useful assay to quantify alterations in cell migratory capacity in response to experimental manipulations. Although several methods exist to study cell migration (such as Boyden chamber assay, barrier assays, and microfluidics-based assays), in this short report we will explain the wound healing assay, also known as the “in vitro scratch assay” as a simple, versatile, and costeffective method to study collective cell migration and wound healing.	es
dc.format	application/pdf
dc.format.extent	6 p.	es
dc.language.iso	eng	es
dc.publisher	Elsevier	es
dc.rights	Aquest document està subjecte a aquesta llicència Creative Commons	es
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/es/	es
dc.subject.other	Ferides i lesions	es
dc.subject.other	Cicatrització	es
dc.title	Research Techniques Made Simple: Analysis of Collective Cell Migration Using the Wound Healing Assay	es
dc.type	info:eu-repo/semantics/article	es
dc.identifier.doi	https://doi.org/10.1016/j.jid.2016.11.020
dc.rights.accessRights	info:eu-repo/semantics/closedAccess	es
dc.type.version	info:eu-repo/publishedVersion	es
dc.indexacio	Indexat a WOS/JCR	es
dc.indexacio	Indexat a SCOPUS	es