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Finding and characterizing Partially Methylated Domains in human haematopoietic cells

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dc.contributor Universitat de Vic. Escola Politècnica Superior
dc.contributor Universitat de Vic. Màster Universitari en Anàlisi de Dades Òmiques
dc.contributor.author Duran Ferrer, Martí
dc.date.accessioned 2014-10-22T09:16:19Z
dc.date.available 2014-10-22T09:16:19Z
dc.date.created 2014-09
dc.date.issued 2014-09
dc.identifier.uri http://hdl.handle.net/10854/3457
dc.description Curs 2013-2014 ca_ES
dc.description.abstract DNA cytosine methylation has been demonstrated to be a central epigenetic modification that has essential roles in a myriad of cellular processes. Some examples of these include gene regulation, DNA-protein interactions, cellular differentiation, X-inactivation, maintenance of genome integrity by suppressing transposable elements and viruses, embryogenesis, genomic imprinting and tumourigenesis. This list is increasingly growing thanks to recent advances in genome-wide technologies, like Whole Genome Bisulfite Sequencing (WGBS-Seq). The development of this technology in research has allowed the identification of new features of the DNA methylation landscape that was not possible using previous technologies, like Partially Methylated Domains (PMDs). PMDs have been found in several cell lines, as well as in both healthy and cancer primary samples. They have been described as regions with high variability in methylation levels across individual CpG sites and intermediate methylation levels on average with respect to the genome. Here, we performed an extensive search of PMDs in a big dataset of different haematopoietic primary cells from both myeloid and lymphoid lineages. We found and characterized significant PMDs in plasma B cells, confirming that PMDs are a phenomenon that is restricted to certain differentiated cells. Additionally, we found loci aberrantly hypomethylated in a myeloma sample which overlapped with plasma B cell PMDs. Genome-wide comparison of the myeloma and plasma B cell sample revealed that this is probably also the case for other loci. ca_ES
dc.format application/pdf
dc.format.extent 19 p. ca_ES
dc.language.iso eng ca_ES
dc.rights Aquest document està subjecte a aquesta llicència Creative Commons ca_ES
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/es/ ca_ES
dc.subject.other ADN ca_ES
dc.subject.other Cèl·lules mare hematopoètiques ca_ES
dc.subject.other Genomes ca_ES
dc.title Finding and characterizing Partially Methylated Domains in human haematopoietic cells ca_ES
dc.type info:eu-repo/semantics/masterThesis ca_ES
dc.description.version Director/a: Simon Heath, Angelika Merkel
dc.rights.accesRights info:eu-repo/semantics/openAccess ca_ES

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