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Comparing assays for the measurement of AhR ligand activity

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dc.contributor Universitat de Vic. Escola Politècnica Superior
dc.contributor.author Planas Botey, Carlota
dc.date.accessioned 2014-09-25T12:22:58Z
dc.date.available 2014-09-25T12:22:58Z
dc.date.created 2014-06-20
dc.date.issued 2014-06-20
dc.identifier.uri http://hdl.handle.net/10854/3293
dc.description Curs 2013-2014 ca_ES
dc.description.abstract In this thesis (TFG) the results of the comparison of three assays for the measurement of AhR ligand activity are exposed. This study was part of a collaborative project aiming at the characterization of the AhR signaling activities of known naturally occurring compounds to explore the potential of using non-toxic compounds to treat inflammatory diseases via oral administration. The first goal of this project was to find an assay able to measure AhR-activity, so the comparison of different assays has been done in order to find the most convenient one according to the efficiency, sensitivity and precision. Moreover, other elements with operational nature such as price, toxicity of components or ease of use has been considered. From the use of compounds known from the literature to be AhR ligands, three assays have been tested: (1) P450-GloTM CYP1A2 Induction/Inhibition assay, (2) quantitative Polymerase Chain Reaction (qPCR) and (3) DR. CALUX® Bioassay. Moreover, a different experiment using the last assay was performed for the study in vivo of the transport of the compounds tested. The results of the TFG suggested the DR. CALUX® Bioassay as the most promising assay to be used for the screening of samples as AhR-ligands because it is quicker, easier to handle and less expensive than qPCR and more reproducible than the CYP1A2 Induction/Inhibition assay. Moreover, the use of this assay allowed having a first idea of which compounds are uptaken by the epithelial barrier and in with direction the transport happens. ca_ES
dc.format application/pdf
dc.format.extent 30 p. ca_ES
dc.language.iso eng ca_ES
dc.rights Aquest document està subjecte a aquesta llicència Creative Commons ca_ES
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/es/ ca_ES
dc.subject.other Bioassaig ca_ES
dc.title Comparing assays for the measurement of AhR ligand activity ca_ES
dc.type info:eu-repo/semantics/bachelorThesis ca_ES
dc.rights.accesRights info:eu-repo/semantics/openAccess ca_ES

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